Journal: Oncology letters

Article Title: HnRNPAB is an independent prognostic factor in non‑small cell lung cancer and is involved in cell proliferation and metastasis.

doi: 10.3892/ol.2023.13801

Figure Lengend Snippet: Figure 3. HnRNPAB‑silencing inhibits the migration, invasion and EMT of NSCLC cells. (A) Cell migration was evaluated using wound healing assay (magnification, x100). (B) Cell invasion was evaluated using Matrigel matrix‑coated Transwell assay (magnification, x100). (C) Protein and mRNA expression levels of EMT‑related markers ZEB1, E‑cadherin, SNAI1, N‑cadherin and Vimentin were determined using western blotting and reverse transcription‑quan‑ titative‑PCR. GAPDH was used as a loading control. For western blotting, multiple membranes from the same sample at the same time were used to expose different target proteins and so >1 GAPDH were provided. Results are representative of three independent experiments. *P<0.05, **P<0.01 and ***P<0.001 compared with respective NC shRNA group. HnRNPAB, heterogeneous nuclear ribonucleoprotein A/B; NC, negative control; shRNA, short hairpin RNA; EMT, epithelial‑mesenchymal transition; ZEB1, zinc finger E‑box binding homeobox 1.

Article Snippet: Rabbit polyclonal antibodies against hnRNPAB (A17497, 1:2,000, ABclonal, Wuhan, China), Glyceraldehyde 3‐phosphate dehydrogenase (GAPDH, 60004‐1‐Ig, 1:5,000, Proteintech, Wuhan, China), ZEB1 (21544‐1‐AP, 1:1,000, Proteintech), N‐cadherin (22018‐1‐AP, 1:5,000, Proteintech), E‐cadherin (20874‐1‐AP, 1:5,000, Proteintech), Vimentin (10366‐1‐AP, 1:10,000, Proteintech) and SNAI1 (13099‐1‐AP, 1:2,000, Proteintech), and a horseradish peroxidase‐conjugated secondary antibody (SA00001‐2, 1:5,000, Proteintech) were used.

Techniques: Migration, Wound Healing Assay, Transwell Assay, Expressing, Western Blot, Control, shRNA, Negative Control, Binding Assay

Journal: iScience

Article Title: FSBP suppresses tumor cell migration by inhibiting the JNK pathway

doi: 10.1016/j.isci.2023.106440

Figure Lengend Snippet: Key resources table

Article Snippet: Primary antibodies were showed as following: mouse anti-Actin (1:5000; Genscript), mouse anti-Fg (1:2000; Sigma), mouse anti-Myc (1:2000; Santa Cruz), rabbit anti-FSBP (1:1000; Atlas antibodies), rabbit anti-E-Cadherin (1:2000; ABclonal), rabbit anti-N-Cadherin (1:1000; ABclonal), rabbit anti-β-Catenin (1:5000; Proteintech), rabbit anti-FAK (1:2000; ABclonal), rabbit anti-Vimentin (1:2000; Proteintech), rabbit anti-Snail (1:2000; ABclonal), rabbit anti-MMP9 (1:1000; ABclonal), rabbit anti-pJNK (1:1000; ABclonal), rabbit anti-phospho-c-Jun (1:1000; ABclonal), rabbit anti-c-Myc (1:1000; ABclonal). secondary antibodies were showed as following: goat anti-rabbit (1:10000; Jackson ImmunoResearch), goat anti-mouse (1:10000; Jackson ImmunoResearch).

Techniques: Recombinant, Protease Inhibitor, Software